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quantitect reverse transcription kit  (Qiagen)


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    Structured Review

    Qiagen quantitect reverse transcription kit
    Quantitect Reverse Transcription Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 18972 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/quantitect%C2%AE+reverse+transcription+kit/Reverse+Transcription+QuantiTect+Probe+RT-PCR+Kit/pm42123637-312-16-20
    Average 99 stars, based on 18972 article reviews
    quantitect reverse transcription kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Synthesized:

    Article Title: Morphological and Biochemical Characteristics of a Novel Albino Tea Cultivar (Camellia sinensis ‘Geumda’)
    Article Snippet: First-strand cDNA was synthesized from 1 μg of total RNA using the QuantiTect® Reverse Transcription kit (Cat. No. 205311, Qiagen, Hilden, Germany).

    Article Title: Promoting Polarization and Differentiation of Primary Human Salivary Gland Stem/Progenitor Cells in Protease-Degradable Hydrogels via ROCK Inhibition.
    Article Snippet: The mRNA was reverse transcribed to cDNA using the QuantiTect® Reverse Transcription Kit (QIAGEN) following the manufacturer's protocol.

    Article Title: Measuring cytokines in Eurasian tundra reindeer (Rangifer tarandus tarandus) with a bovine bead-based multiplex immunoassay and real-time PCR.
    Article Snippet: Diluted total RNA (20 μg/mL) was reverse transcribed into complementary DNA (cDNA) using the QuantiTect® Reverse Transcription Kit (Qiagen) and Veriti 96-Well Fast Thermal Cycler (Thermo Fisher Scientific) including a genomic DNA elimination reaction following the manufacturer’s protocol.

    Article Title: Impact of antibacterial therapeutic agents on biofilm-tissue interactions in a 3D implant-tissue-oral-bacterial-biofilm model.
    Article Snippet: A total of 500 ng of RNA was reverse transcribed with included DNase digestion using the QuantiTect® Reverse Transcription Kit (205311, Qiagen) in accordance with the manufacturer’s protocol.

    Article Title: The efficacy of nanoparticles on soil microbial biodiversity and the prevention of Fusarium wilt disease (Fusarium oxysporum f.sp. lycopersici).
    Article Snippet: The isolated RNA was processed using the QuantiTect® Reverse Transcription Kit (Qiagen, Cat. No. 205,311) to produce complementary DNA (cDNA).

    Article Title: Integrated Analysis of Long Non-Coding RNAs and mRNAs Reveals Key Trans-Target Genes Associated with Heat Stress Response in Rhododendron delavayi .
    Article Snippet: The mRNA was reverse-transcribed to the first-strand cDNA using QuantiTect® Reverse Transcription kit (Qiagen, Hilden, Germany).

    Article Title: Hypoxia-Dependent Upregulation of VEGF Relies on β3-Adrenoceptor Signaling in Human Retinal Endothelial and Müller Cells.
    Article Snippet: Before cDNA synthesis, RNA samples were also tested for the absence of genomic DNA. cDNA was synthesized starting from 1 μg of total RNA using a commercially available kit (QuantiTect® Reverse Transcription Kit; Qiagen). qRT-PCR analysis was performed using a master mix (SsoAdvanced Universal SYBR Green Supermix; Bio-Rad Laboratories, Inc.) on a detection system (CFX Connect Real-Time PCR Detection System provided with the software CFX manager version 3.1; Bio-Rad Laboratories, Inc.).

    Article Title: Ti 3 C 2 T x MXene augments osmo-adaptive repression of the inflammatory stress response for improved wound repair.
    Article Snippet: Genomic DNA was removed, and cDNA synthesis was performed using a QuantiTect® Reverse Transcription Kit (Qiagen, UK).

    Reverse Transcription:

    Article Title: Morphological and Biochemical Characteristics of a Novel Albino Tea Cultivar (Camellia sinensis ‘Geumda’)
    Article Snippet: First-strand cDNA was synthesized from 1 μg of total RNA using the QuantiTect® Reverse Transcription kit (Cat. No. 205311, Qiagen, Hilden, Germany).

    Article Title: Promoting Polarization and Differentiation of Primary Human Salivary Gland Stem/Progenitor Cells in Protease-Degradable Hydrogels via ROCK Inhibition.
    Article Snippet: The mRNA was reverse transcribed to cDNA using the QuantiTect® Reverse Transcription Kit (QIAGEN) following the manufacturer's protocol.

    Article Title: Measuring cytokines in Eurasian tundra reindeer (Rangifer tarandus tarandus) with a bovine bead-based multiplex immunoassay and real-time PCR.
    Article Snippet: Diluted total RNA (20 μg/mL) was reverse transcribed into complementary DNA (cDNA) using the QuantiTect® Reverse Transcription Kit (Qiagen) and Veriti 96-Well Fast Thermal Cycler (Thermo Fisher Scientific) including a genomic DNA elimination reaction following the manufacturer’s protocol.

    Article Title: Impact of antibacterial therapeutic agents on biofilm-tissue interactions in a 3D implant-tissue-oral-bacterial-biofilm model.
    Article Snippet: A total of 500 ng of RNA was reverse transcribed with included DNase digestion using the QuantiTect® Reverse Transcription Kit (205311, Qiagen) in accordance with the manufacturer’s protocol.

    Article Title: The efficacy of nanoparticles on soil microbial biodiversity and the prevention of Fusarium wilt disease (Fusarium oxysporum f.sp. lycopersici).
    Article Snippet: The isolated RNA was processed using the QuantiTect® Reverse Transcription Kit (Qiagen, Cat. No. 205,311) to produce complementary DNA (cDNA).

    Article Title: Integrated Analysis of Long Non-Coding RNAs and mRNAs Reveals Key Trans-Target Genes Associated with Heat Stress Response in Rhododendron delavayi .
    Article Snippet: The mRNA was reverse-transcribed to the first-strand cDNA using QuantiTect® Reverse Transcription kit (Qiagen, Hilden, Germany).

    Article Title: Hypoxia-Dependent Upregulation of VEGF Relies on β3-Adrenoceptor Signaling in Human Retinal Endothelial and Müller Cells.
    Article Snippet: Before cDNA synthesis, RNA samples were also tested for the absence of genomic DNA. cDNA was synthesized starting from 1 μg of total RNA using a commercially available kit (QuantiTect® Reverse Transcription Kit; Qiagen). qRT-PCR analysis was performed using a master mix (SsoAdvanced Universal SYBR Green Supermix; Bio-Rad Laboratories, Inc.) on a detection system (CFX Connect Real-Time PCR Detection System provided with the software CFX manager version 3.1; Bio-Rad Laboratories, Inc.).

    Article Title: Ti 3 C 2 T x MXene augments osmo-adaptive repression of the inflammatory stress response for improved wound repair.
    Article Snippet: Genomic DNA was removed, and cDNA synthesis was performed using a QuantiTect® Reverse Transcription Kit (Qiagen, UK).

    Isolation:

    Article Title: Morphological and Biochemical Characteristics of a Novel Albino Tea Cultivar (Camellia sinensis ‘Geumda’)
    Article Snippet: First-strand cDNA was synthesized from 1 μg of total RNA using the QuantiTect® Reverse Transcription kit (Cat. No. 205311, Qiagen, Hilden, Germany).

    Article Title: Promoting Polarization and Differentiation of Primary Human Salivary Gland Stem/Progenitor Cells in Protease-Degradable Hydrogels via ROCK Inhibition.
    Article Snippet: The mRNA was reverse transcribed to cDNA using the QuantiTect® Reverse Transcription Kit (QIAGEN) following the manufacturer's protocol.

    Article Title: Measuring cytokines in Eurasian tundra reindeer (Rangifer tarandus tarandus) with a bovine bead-based multiplex immunoassay and real-time PCR.
    Article Snippet: Diluted total RNA (20 μg/mL) was reverse transcribed into complementary DNA (cDNA) using the QuantiTect® Reverse Transcription Kit (Qiagen) and Veriti 96-Well Fast Thermal Cycler (Thermo Fisher Scientific) including a genomic DNA elimination reaction following the manufacturer’s protocol.

    Article Title: Impact of antibacterial therapeutic agents on biofilm-tissue interactions in a 3D implant-tissue-oral-bacterial-biofilm model.
    Article Snippet: A total of 500 ng of RNA was reverse transcribed with included DNase digestion using the QuantiTect® Reverse Transcription Kit (205311, Qiagen) in accordance with the manufacturer’s protocol.

    Article Title: The efficacy of nanoparticles on soil microbial biodiversity and the prevention of Fusarium wilt disease (Fusarium oxysporum f.sp. lycopersici).
    Article Snippet: The isolated RNA was processed using the QuantiTect® Reverse Transcription Kit (Qiagen, Cat. No. 205,311) to produce complementary DNA (cDNA).

    Article Title: Integrated Analysis of Long Non-Coding RNAs and mRNAs Reveals Key Trans-Target Genes Associated with Heat Stress Response in Rhododendron delavayi .
    Article Snippet: The mRNA was reverse-transcribed to the first-strand cDNA using QuantiTect® Reverse Transcription kit (Qiagen, Hilden, Germany).

    Article Title: Hypoxia-Dependent Upregulation of VEGF Relies on β3-Adrenoceptor Signaling in Human Retinal Endothelial and Müller Cells.
    Article Snippet: Before cDNA synthesis, RNA samples were also tested for the absence of genomic DNA. cDNA was synthesized starting from 1 μg of total RNA using a commercially available kit (QuantiTect® Reverse Transcription Kit; Qiagen). qRT-PCR analysis was performed using a master mix (SsoAdvanced Universal SYBR Green Supermix; Bio-Rad Laboratories, Inc.) on a detection system (CFX Connect Real-Time PCR Detection System provided with the software CFX manager version 3.1; Bio-Rad Laboratories, Inc.).

    Article Title: Ti 3 C 2 T x MXene augments osmo-adaptive repression of the inflammatory stress response for improved wound repair.
    Article Snippet: Genomic DNA was removed, and cDNA synthesis was performed using a QuantiTect® Reverse Transcription Kit (Qiagen, UK).

    cDNA Synthesis:

    Article Title: Morphological and Biochemical Characteristics of a Novel Albino Tea Cultivar (Camellia sinensis ‘Geumda’)
    Article Snippet: First-strand cDNA was synthesized from 1 μg of total RNA using the QuantiTect® Reverse Transcription kit (Cat. No. 205311, Qiagen, Hilden, Germany).

    Article Title: Promoting Polarization and Differentiation of Primary Human Salivary Gland Stem/Progenitor Cells in Protease-Degradable Hydrogels via ROCK Inhibition.
    Article Snippet: The mRNA was reverse transcribed to cDNA using the QuantiTect® Reverse Transcription Kit (QIAGEN) following the manufacturer's protocol.

    Article Title: Measuring cytokines in Eurasian tundra reindeer (Rangifer tarandus tarandus) with a bovine bead-based multiplex immunoassay and real-time PCR.
    Article Snippet: Diluted total RNA (20 μg/mL) was reverse transcribed into complementary DNA (cDNA) using the QuantiTect® Reverse Transcription Kit (Qiagen) and Veriti 96-Well Fast Thermal Cycler (Thermo Fisher Scientific) including a genomic DNA elimination reaction following the manufacturer’s protocol.

    Article Title: Impact of antibacterial therapeutic agents on biofilm-tissue interactions in a 3D implant-tissue-oral-bacterial-biofilm model.
    Article Snippet: A total of 500 ng of RNA was reverse transcribed with included DNase digestion using the QuantiTect® Reverse Transcription Kit (205311, Qiagen) in accordance with the manufacturer’s protocol.

    Article Title: The efficacy of nanoparticles on soil microbial biodiversity and the prevention of Fusarium wilt disease (Fusarium oxysporum f.sp. lycopersici).
    Article Snippet: The isolated RNA was processed using the QuantiTect® Reverse Transcription Kit (Qiagen, Cat. No. 205,311) to produce complementary DNA (cDNA).

    Article Title: Integrated Analysis of Long Non-Coding RNAs and mRNAs Reveals Key Trans-Target Genes Associated with Heat Stress Response in Rhododendron delavayi .
    Article Snippet: The mRNA was reverse-transcribed to the first-strand cDNA using QuantiTect® Reverse Transcription kit (Qiagen, Hilden, Germany).

    Article Title: Hypoxia-Dependent Upregulation of VEGF Relies on β3-Adrenoceptor Signaling in Human Retinal Endothelial and Müller Cells.
    Article Snippet: Before cDNA synthesis, RNA samples were also tested for the absence of genomic DNA. cDNA was synthesized starting from 1 μg of total RNA using a commercially available kit (QuantiTect® Reverse Transcription Kit; Qiagen). qRT-PCR analysis was performed using a master mix (SsoAdvanced Universal SYBR Green Supermix; Bio-Rad Laboratories, Inc.) on a detection system (CFX Connect Real-Time PCR Detection System provided with the software CFX manager version 3.1; Bio-Rad Laboratories, Inc.).

    Article Title: Ti 3 C 2 T x MXene augments osmo-adaptive repression of the inflammatory stress response for improved wound repair.
    Article Snippet: Genomic DNA was removed, and cDNA synthesis was performed using a QuantiTect® Reverse Transcription Kit (Qiagen, UK).

    Quantitative RT-PCR:

    Article Title: Morphological and Biochemical Characteristics of a Novel Albino Tea Cultivar (Camellia sinensis ‘Geumda’)
    Article Snippet: First-strand cDNA was synthesized from 1 μg of total RNA using the QuantiTect® Reverse Transcription kit (Cat. No. 205311, Qiagen, Hilden, Germany).

    Article Title: Promoting Polarization and Differentiation of Primary Human Salivary Gland Stem/Progenitor Cells in Protease-Degradable Hydrogels via ROCK Inhibition.
    Article Snippet: The mRNA was reverse transcribed to cDNA using the QuantiTect® Reverse Transcription Kit (QIAGEN) following the manufacturer's protocol.

    Article Title: Measuring cytokines in Eurasian tundra reindeer (Rangifer tarandus tarandus) with a bovine bead-based multiplex immunoassay and real-time PCR.
    Article Snippet: Diluted total RNA (20 μg/mL) was reverse transcribed into complementary DNA (cDNA) using the QuantiTect® Reverse Transcription Kit (Qiagen) and Veriti 96-Well Fast Thermal Cycler (Thermo Fisher Scientific) including a genomic DNA elimination reaction following the manufacturer’s protocol.

    Article Title: Impact of antibacterial therapeutic agents on biofilm-tissue interactions in a 3D implant-tissue-oral-bacterial-biofilm model.
    Article Snippet: A total of 500 ng of RNA was reverse transcribed with included DNase digestion using the QuantiTect® Reverse Transcription Kit (205311, Qiagen) in accordance with the manufacturer’s protocol.

    Article Title: The efficacy of nanoparticles on soil microbial biodiversity and the prevention of Fusarium wilt disease (Fusarium oxysporum f.sp. lycopersici).
    Article Snippet: The isolated RNA was processed using the QuantiTect® Reverse Transcription Kit (Qiagen, Cat. No. 205,311) to produce complementary DNA (cDNA).

    Article Title: Integrated Analysis of Long Non-Coding RNAs and mRNAs Reveals Key Trans-Target Genes Associated with Heat Stress Response in Rhododendron delavayi .
    Article Snippet: The mRNA was reverse-transcribed to the first-strand cDNA using QuantiTect® Reverse Transcription kit (Qiagen, Hilden, Germany).

    Article Title: Hypoxia-Dependent Upregulation of VEGF Relies on β3-Adrenoceptor Signaling in Human Retinal Endothelial and Müller Cells.
    Article Snippet: Before cDNA synthesis, RNA samples were also tested for the absence of genomic DNA. cDNA was synthesized starting from 1 μg of total RNA using a commercially available kit (QuantiTect® Reverse Transcription Kit; Qiagen). qRT-PCR analysis was performed using a master mix (SsoAdvanced Universal SYBR Green Supermix; Bio-Rad Laboratories, Inc.) on a detection system (CFX Connect Real-Time PCR Detection System provided with the software CFX manager version 3.1; Bio-Rad Laboratories, Inc.).

    Article Title: Ti 3 C 2 T x MXene augments osmo-adaptive repression of the inflammatory stress response for improved wound repair.
    Article Snippet: Genomic DNA was removed, and cDNA synthesis was performed using a QuantiTect® Reverse Transcription Kit (Qiagen, UK).

    SYBR Green Assay:

    Article Title: Morphological and Biochemical Characteristics of a Novel Albino Tea Cultivar (Camellia sinensis ‘Geumda’)
    Article Snippet: First-strand cDNA was synthesized from 1 μg of total RNA using the QuantiTect® Reverse Transcription kit (Cat. No. 205311, Qiagen, Hilden, Germany).

    Article Title: Promoting Polarization and Differentiation of Primary Human Salivary Gland Stem/Progenitor Cells in Protease-Degradable Hydrogels via ROCK Inhibition.
    Article Snippet: The mRNA was reverse transcribed to cDNA using the QuantiTect® Reverse Transcription Kit (QIAGEN) following the manufacturer's protocol.

    Article Title: Measuring cytokines in Eurasian tundra reindeer (Rangifer tarandus tarandus) with a bovine bead-based multiplex immunoassay and real-time PCR.
    Article Snippet: Diluted total RNA (20 μg/mL) was reverse transcribed into complementary DNA (cDNA) using the QuantiTect® Reverse Transcription Kit (Qiagen) and Veriti 96-Well Fast Thermal Cycler (Thermo Fisher Scientific) including a genomic DNA elimination reaction following the manufacturer’s protocol.

    Article Title: Impact of antibacterial therapeutic agents on biofilm-tissue interactions in a 3D implant-tissue-oral-bacterial-biofilm model.
    Article Snippet: A total of 500 ng of RNA was reverse transcribed with included DNase digestion using the QuantiTect® Reverse Transcription Kit (205311, Qiagen) in accordance with the manufacturer’s protocol.

    Article Title: The efficacy of nanoparticles on soil microbial biodiversity and the prevention of Fusarium wilt disease (Fusarium oxysporum f.sp. lycopersici).
    Article Snippet: The isolated RNA was processed using the QuantiTect® Reverse Transcription Kit (Qiagen, Cat. No. 205,311) to produce complementary DNA (cDNA).

    Article Title: Integrated Analysis of Long Non-Coding RNAs and mRNAs Reveals Key Trans-Target Genes Associated with Heat Stress Response in Rhododendron delavayi .
    Article Snippet: The mRNA was reverse-transcribed to the first-strand cDNA using QuantiTect® Reverse Transcription kit (Qiagen, Hilden, Germany).

    Article Title: Hypoxia-Dependent Upregulation of VEGF Relies on β3-Adrenoceptor Signaling in Human Retinal Endothelial and Müller Cells.
    Article Snippet: Before cDNA synthesis, RNA samples were also tested for the absence of genomic DNA. cDNA was synthesized starting from 1 μg of total RNA using a commercially available kit (QuantiTect® Reverse Transcription Kit; Qiagen). qRT-PCR analysis was performed using a master mix (SsoAdvanced Universal SYBR Green Supermix; Bio-Rad Laboratories, Inc.) on a detection system (CFX Connect Real-Time PCR Detection System provided with the software CFX manager version 3.1; Bio-Rad Laboratories, Inc.).

    Article Title: Ti 3 C 2 T x MXene augments osmo-adaptive repression of the inflammatory stress response for improved wound repair.
    Article Snippet: Genomic DNA was removed, and cDNA synthesis was performed using a QuantiTect® Reverse Transcription Kit (Qiagen, UK).

    Real-time Polymerase Chain Reaction:

    Article Title: Morphological and Biochemical Characteristics of a Novel Albino Tea Cultivar (Camellia sinensis ‘Geumda’)
    Article Snippet: First-strand cDNA was synthesized from 1 μg of total RNA using the QuantiTect® Reverse Transcription kit (Cat. No. 205311, Qiagen, Hilden, Germany).

    Article Title: Promoting Polarization and Differentiation of Primary Human Salivary Gland Stem/Progenitor Cells in Protease-Degradable Hydrogels via ROCK Inhibition.
    Article Snippet: The mRNA was reverse transcribed to cDNA using the QuantiTect® Reverse Transcription Kit (QIAGEN) following the manufacturer's protocol.

    Article Title: Measuring cytokines in Eurasian tundra reindeer (Rangifer tarandus tarandus) with a bovine bead-based multiplex immunoassay and real-time PCR.
    Article Snippet: Diluted total RNA (20 μg/mL) was reverse transcribed into complementary DNA (cDNA) using the QuantiTect® Reverse Transcription Kit (Qiagen) and Veriti 96-Well Fast Thermal Cycler (Thermo Fisher Scientific) including a genomic DNA elimination reaction following the manufacturer’s protocol.

    Article Title: Impact of antibacterial therapeutic agents on biofilm-tissue interactions in a 3D implant-tissue-oral-bacterial-biofilm model.
    Article Snippet: A total of 500 ng of RNA was reverse transcribed with included DNase digestion using the QuantiTect® Reverse Transcription Kit (205311, Qiagen) in accordance with the manufacturer’s protocol.

    Article Title: The efficacy of nanoparticles on soil microbial biodiversity and the prevention of Fusarium wilt disease (Fusarium oxysporum f.sp. lycopersici).
    Article Snippet: The isolated RNA was processed using the QuantiTect® Reverse Transcription Kit (Qiagen, Cat. No. 205,311) to produce complementary DNA (cDNA).

    Article Title: Integrated Analysis of Long Non-Coding RNAs and mRNAs Reveals Key Trans-Target Genes Associated with Heat Stress Response in Rhododendron delavayi .
    Article Snippet: The mRNA was reverse-transcribed to the first-strand cDNA using QuantiTect® Reverse Transcription kit (Qiagen, Hilden, Germany).

    Article Title: Hypoxia-Dependent Upregulation of VEGF Relies on β3-Adrenoceptor Signaling in Human Retinal Endothelial and Müller Cells.
    Article Snippet: Before cDNA synthesis, RNA samples were also tested for the absence of genomic DNA. cDNA was synthesized starting from 1 μg of total RNA using a commercially available kit (QuantiTect® Reverse Transcription Kit; Qiagen). qRT-PCR analysis was performed using a master mix (SsoAdvanced Universal SYBR Green Supermix; Bio-Rad Laboratories, Inc.) on a detection system (CFX Connect Real-Time PCR Detection System provided with the software CFX manager version 3.1; Bio-Rad Laboratories, Inc.).

    Article Title: Ti 3 C 2 T x MXene augments osmo-adaptive repression of the inflammatory stress response for improved wound repair.
    Article Snippet: Genomic DNA was removed, and cDNA synthesis was performed using a QuantiTect® Reverse Transcription Kit (Qiagen, UK).

    Software:

    Article Title: Morphological and Biochemical Characteristics of a Novel Albino Tea Cultivar (Camellia sinensis ‘Geumda’)
    Article Snippet: First-strand cDNA was synthesized from 1 μg of total RNA using the QuantiTect® Reverse Transcription kit (Cat. No. 205311, Qiagen, Hilden, Germany).

    Article Title: Promoting Polarization and Differentiation of Primary Human Salivary Gland Stem/Progenitor Cells in Protease-Degradable Hydrogels via ROCK Inhibition.
    Article Snippet: The mRNA was reverse transcribed to cDNA using the QuantiTect® Reverse Transcription Kit (QIAGEN) following the manufacturer's protocol.

    Article Title: Measuring cytokines in Eurasian tundra reindeer (Rangifer tarandus tarandus) with a bovine bead-based multiplex immunoassay and real-time PCR.
    Article Snippet: Diluted total RNA (20 μg/mL) was reverse transcribed into complementary DNA (cDNA) using the QuantiTect® Reverse Transcription Kit (Qiagen) and Veriti 96-Well Fast Thermal Cycler (Thermo Fisher Scientific) including a genomic DNA elimination reaction following the manufacturer’s protocol.

    Article Title: Impact of antibacterial therapeutic agents on biofilm-tissue interactions in a 3D implant-tissue-oral-bacterial-biofilm model.
    Article Snippet: A total of 500 ng of RNA was reverse transcribed with included DNase digestion using the QuantiTect® Reverse Transcription Kit (205311, Qiagen) in accordance with the manufacturer’s protocol.

    Article Title: The efficacy of nanoparticles on soil microbial biodiversity and the prevention of Fusarium wilt disease (Fusarium oxysporum f.sp. lycopersici).
    Article Snippet: The isolated RNA was processed using the QuantiTect® Reverse Transcription Kit (Qiagen, Cat. No. 205,311) to produce complementary DNA (cDNA).

    Article Title: Integrated Analysis of Long Non-Coding RNAs and mRNAs Reveals Key Trans-Target Genes Associated with Heat Stress Response in Rhododendron delavayi .
    Article Snippet: The mRNA was reverse-transcribed to the first-strand cDNA using QuantiTect® Reverse Transcription kit (Qiagen, Hilden, Germany).

    Article Title: Hypoxia-Dependent Upregulation of VEGF Relies on β3-Adrenoceptor Signaling in Human Retinal Endothelial and Müller Cells.
    Article Snippet: Before cDNA synthesis, RNA samples were also tested for the absence of genomic DNA. cDNA was synthesized starting from 1 μg of total RNA using a commercially available kit (QuantiTect® Reverse Transcription Kit; Qiagen). qRT-PCR analysis was performed using a master mix (SsoAdvanced Universal SYBR Green Supermix; Bio-Rad Laboratories, Inc.) on a detection system (CFX Connect Real-Time PCR Detection System provided with the software CFX manager version 3.1; Bio-Rad Laboratories, Inc.).

    Article Title: Ti 3 C 2 T x MXene augments osmo-adaptive repression of the inflammatory stress response for improved wound repair.
    Article Snippet: Genomic DNA was removed, and cDNA synthesis was performed using a QuantiTect® Reverse Transcription Kit (Qiagen, UK).



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